Polychlorinated biphenyl 153 exacerbates nonalcoholic fatty liver disease in C57BL/6 mice

POLYCHLORINATED BIPHENYL 153 EXACERBATES NONALCOHOLIC FATTY LIVER DISEASE IN C57BU6 MICE Banrida Wahlang April 04,2012 Polychlorinated biphenyls (PCBs) are persistent environmental pollutants which are detectable in the serum of all American adults. Amongst PCB congeners, PCB 153 has the highest serum level. PCBs have been dose-dependently associated with suspected nonalcoholic fatty liver disease (NAFLD), obesity and metabolic syndrome in epidemiological studies. The purpose of this study is to determine if PCB 153 induces NAFLD in mice fed a control diet (CD), and exacerbates NAFLD in mice fed a high fat diet (HFD). C57BL6/J mice were fed either control or 42% milk fat diet for 12 weeks with or without PCB 153 coexposure (50 mg/kg i.p. x 4). Glucose tolerance tests were performed, and plasma/tissues were obtained at necropsy for measurements of adipocytokine levels, histology, and gene expression microarrays. In mice fed CD, the addition of PCB 153 had little to no effect on any of the measured parameters. In contrast, PCB 153 co-exposure in high fat-fed mice was associated with dramatically increased visceral adiposity, hepatic steatosis and increased plasma adipokines